A depiction of a 28-day menstrual cycle with ovulation on day 14, showing the typical fluctuations in ovarian hormones, such as oestrogen, progesterone and luteinising hormone (LH), as well as the changes in temperature, during the follicular and luteal phases. Modified from Peinado et al20 and Schmalenberger et al.28 Timing of sessions for an average duration cycle (28 days): from the first day of bleeding, the tests will be performed between the first and fifth day (EFP=low levels of sex hormones), between days 11 and 13 (LH peak or the same day; LFP=low levels of progesterone but high oestrogen levels), and between days 19 and 23 (after ovulation; MLP=elevated levels of both progesterone and oestrogen). EFP, early follicular phase; LFP, late follicular phase; MLP, mid-luteal phase.
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