An ergometer, to be used for resistance training in space, has been developed and validated. It is designed to activate the extensor muscles of the knee and ankle joints while performing the leg press exercise. Resistance is provided independent of gravity by using the inertial focus of a flywheel. Eleven men performed two series of consecutive maximal voluntary concentric and eccentric muscle actions. Force, power, work and electromyographic (EMG) activity, measured during exercise on this ergometer and a traditional leg press resistive apparatus were similar. This mechanical ergometer seems to meet the operational and technical requirements of equipment that can be flown and used in space. Also, the physiological responses to acute exercise suggest that adaptations similar to those achieved by traditional weight training can be produced. Exercise using the inertia ergometer would, therefore, probably also be effective in combating the muscle atrophy and loss of strength that occur in microgravity.