In this study, we investigated the relationship between physiological, anthropometric, and skill qualities and playing performance in professional rugby league players. Fifty-eight high-performance rugby league players underwent measurements for anthropometry (height, body mass, sum of seven skinfolds), physiological (speed, change of direction speed, lower body muscular power, repeated-sprint ability, prolonged high-intensity intermittent running ability, and estimated maximal aerobic power), technical skill (tackling proficiency, draw and pass proficiency), and perceptual skill (reactive agility, pattern recall, pattern prediction) qualities. National Rugby League matches were coded for attacking (e.g. line breaks, try assists, etc.) and defensive (e.g. missed tackles, tackling efficiency, etc.) statistics commonly used to assess rugby league playing performance. The number of line break assists was significantly associated (P < 0.05) with greater playing experience (r = 0.36), dual-task draw and pass proficiency (r = 0.54), reactive agility (r = 0.29), and pattern recall (r = 0.32) and prediction (r = 0.28) ability, while faster speed over 40 m (r = -0.42) was associated (P < 0.05) with a higher number of tries scored. Greater age and playing experience, better lower body muscular power, and faster 10 m and 40 m speed were significantly associated (P < 0.05) with the number of tackle attempts (positive), tackles completed (positive), and proportion of missed tackles (negative). These findings demonstrate that well-developed physical and skill qualities are associated with effective playing performance in National Rugby League players.