Arthroscopy: The Journal of Arthroscopic & Related Surgery
Original ArticleCharacterization and Comparison of 5 Platelet-Rich Plasma Preparations in a Single-Donor Model
Section snippets
Different PRP Preparation Systems
Four commercial PRP separation systems and 1 preparation developed in our laboratory were selected for the study. The commercial preparations were the Selphyl System (Selphyl, Bethlehem, PA) and RegenPRP (RegenLab, Le Mont-sur-Lausanne, Switzerland), both using a gel separator system; the Mini GPS III System (Biomet Biology, Warsaw, IN), using a floating buoy separator system; and Arthrex ACP (Arthrex, Naples, FL), using a double-syringe system. The preparation developed in our laboratory was
Whole Blood Characteristics
Table 2 summarizes mean values of platelet and WBC concentrations and the relative composition of whole blood from the 10 healthy donors, which were all within the ranges of normal biological values.
Volume of PRP After Processing
The greatest volumes of PRP were obtained from the Selphyl System and Arthrex ACP, with 4.1 ± 0.43 mL and 4.03 ± 0.35 mL, respectively. ANOVA showed significant differences among the different systems (P = .0002) (Table 3), and pairwise analysis confirmed that the Selphyl System and Arthrex ACP
Discussion
As expected, significant differences have been highlighted among the different PRP preparations, which could explain the large variability in the clinical benefit of PRP reported in the literature and support a minimal characterization of PRP before injection. Thus our study supports the results of the meta-analysis by Sheth et al.29 that showed a large variability in the results obtained from PRP applications in the orthopaedic domain.
However, our approach differs from comparable studies in
Conclusions
In a single-donor model, significant biological variations in PRP obtained from different preparation systems were highlighted. The observed differences suggest different results for treated tissue and could explain the large variability in the clinical benefit of PRP reported in the literature. Our findings will help clinicians to choose a system that meets their specific needs for a given indication.
References (41)
- et al.
Platelet gel: An autologous alternative to fibrin glue with applications in oral and maxillofacial surgery
J Oral Maxillofac Surg
(1997) - et al.
The use of platelet-rich plasma in arthroscopy and sports medicine: Optimizing the healing environment
Arthroscopy
(2010) - et al.
What is platelet-rich plasma?
Oper Tech Sports Med
(2011) - et al.
Comparison of magnetic resonance imaging findings in anterior cruciate ligament grafts with and without autologous platelet-derived growth factors
Arthroscopy
(2010) - et al.
Contents and formulations of platelet-rich plasma
Oper Tech Orthop
(2012) - et al.
The effect of anticoagulants on the quality and biological efficacy of platelet-rich plasma
Clin Biochem
(2009) - et al.
Platelet-rich plasma: The PAW classification system
Arthroscopy
(2012) - et al.
Effect of platelet concentration in platelet-rich plasma on peri-implant bone regeneration
Bone
(2004) - et al.
Poor standardization in platelet-rich therapies hampers advancement
Arthroscopy
(2010) - et al.
Classification of platelet concentrates: From pure platelet-rich plasma (P-PRP) to leucocyte- and platelet-rich fibrin (L-PRF)
Trends Biotechnol
(2009)
Granules of the human neutrophilic polymorphonuclear leukocyte
Blood
Involvement of platelets in stimulating osteogenic activity
J Orthop Res
Platelet-rich plasma (PRP): What is PRP and what is not PRP?
Implant Dent
Regulation of wound healing by growth factors and cytokines
Physiol Rev
Platelet-rich plasma peptides: Key for regeneration
Int J Pept
Formulation and storage of platelet-rich plasma homemade product
Biores Open Access
Intra-articular injection of an autologous preparation rich in growth factors for the treatment of knee OA: A retrospective cohort study
Clin Exp Rheumatol
Efficacy of platelet gel in reconstructive bone surgery
Orthopedics
Do autologous growth factors enhance transforaminal lumbar interbody fusion?
Eur Spine J
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The authors report that they have no conflicts of interest in the authorship and publication of this article.