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The effect of 1 week of repeated ischaemic leg preconditioning on simulated Keirin cycling performance: a randomised trial
  1. Angus Lindsay1,2,
  2. Carl Petersen1,
  3. Gavin Blackwell1,
  4. Hamish Ferguson3,
  5. Greg Parker4,
  6. Nina Steyn4,
  7. Steven P Gieseg4,5
  1. 1 School of Health Sciences, University of Canterbury, Christchurch, New Zealand
  2. 2 Program in Physical Therapy and Rehabilitation Sciences, University of Minnesota, Minneapolis, USA
  3. 3 Sport Performance Research Institute, Auckland University of Technology, Auckland, New Zealand
  4. 4 School of Biological Sciences, University of Canterbury College of Science, Christchurch, New Zealand
  5. 5 Department of Radiology, University of Otago Christchurch, Christchurch, New Zealand
  1. Correspondence to Dr Carl Petersen; carl.petersen{at}


Background Coaches continually seek new ways of doing things and also refine existing techniques to improve sporting performance. Coaches are currently experimenting using ischaemic preconditioning (IPC) over consecutive days in the hope of improving competitive performances.

Aims First, to quantify the physiological impact of 1 week of IPC on simulated Keirin cycling performance. Second, to investigate if biochemical stress markers are affected over the treatment period.

Methods Using a randomised, sham-controlled design, 18 active adults undertook seven consecutive days of IPC treatment (4×5 min occlusion/reperfusion) applied to each leg at either 220 mm Hg (treatment, n=9) or 20 mm Hg (sham, n=9). Urinary measures of inflammation, oxidative stress and indirect nitric oxide synthesis were undertaken daily. A simulated Keirin cycling competition (4×30 s Wingate tests) was performed on day 10, with baseline and postintervention cycling VO2max (days 1, 11 and 18) and 30 s Wingate tests (day 2) undertaken for comparison.

Results The treatment group had enhanced mean cycling power (3.4%), while neopterin and biopterin in conjunction with total neopterin were significantly lower (p<0.05) and total biopterin significantly greater (p<0.05) during the simulated Keirin. Aerobic fitness measures significantly improved from baseline to postintervention (VO2peak: 12.8% ↑, maximal aerobic power: 18.5% ↑).

Conclusions Seven consecutive days of IPC improved aerobic and anaerobic capacity measures, with modulations in oxidative stress, immune system activation and nitric oxide/catecholamine synthesis.

  • biopterin
  • inflammation
  • oxidative stress
  • neopterin

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Coaches seek new ways to improve sporting performance, and considerable research has focused on pre-exercise interventions.1 2 Repetition of using pressure to induce brief ischaemia (hypoxia) followed by reperfusion (reoxygenation) is known as ischaemic preconditioning (IPC). This technique can increase exercise capacity through alterations in oxygen delivery, blood flow and fatigue attenuation,3 and results in improved peak oxygen uptake4 and peak and mean power during the early stages of repeated sprint cycling.5 There is currently insufficient experimental proof to draw confident conclusions about the potential of IPC to acutely enhance sports performance,6 and this may be explained by respondents and non-respondents.6 If a single episode of IPC does enhance performance, would repeated exposure over several days induce an even greater effect? One recent study repeated IPC over nine consecutive days but failed to see any improvement in aerobic capacity.7 Repeated IPC has been investigated in clinical studies, with local and remote improvements in endothelial function, brachial artery flow-mediated dilation and resting skin microcirculation being observed,8–10 although the physiological basis of this ‘hyperconditioning’ is still to be fully elucidated.11

Oxidative stress is an integral component of ischaemia/reperfusion,12 while vasodilation improved tissue oxygenation,13 14 and inflammatory system modulation15 is regulated through single or repetitive ischaemic treatment. Neopterin, 7,8-dihydroneopterin, biopterin, 7,8-dihydrobiopterin and tetrahydrobiopterin are heterocyclic pterin compounds metabolised by various cell types whose detection and elevation represent perturbations in oxidative stress, immune system activation and nitric oxide/catecholamine synthesis.16–21 Collectively, measurement of these biomarkers should provide some physiological insight into the stress response associated with repeated IPC.

Our research purpose is to assess induced physiological changes and simulated Keirin cycling performance following 7 days of IPC, and seek to determine if any aerobic capacity changes remain after an additional 7 days. We also aim to determine if urinary pterins are affected by the repeated ischaemia-reperfusion tissue exposure during treatment as well as during exercise.



Eighteen recreationally active (exercising 5.1±1.7 hours/week) sport science students (13 men, 5 women; age: 23.2±7.1 year; height: 177±11.4 cm; weight: 79.2±12.7 kg; lean body mass: 62.4±11.4 kg; VO2max: 38.6±5.9 mL/min/kg) volunteered for the study. All procedures performed were in accordance with the ethical standards of the University of Canterbury Human Ethics Committee. Informed consent was obtained from all individuals.

Experimental design

A randomised, sham-controlled, single-blinded (researchers only) design was used to assess the impact of seven consecutive days of IPC sessions on urinary markers of physiological stress and simulated competitive cycling performance measured through repeated anaerobic exercise performances. A power analysis for urinary pterins indicated that we needed nine or more subjects using a power of 80 and p value <0.05.

Baseline aerobic (VO2max) and anaerobic capacity (30 s Wingate) tests were performed 48 and 24 hours, respectively, prior to commencing the IPC protocol. Using relative VO2max, participants were pair-matched by the lead investigator, and using a coin toss were randomly allocated into treatment or ‘sham’ group. The participants were told that it was an IPC study using blood pressure cuffs; however, they were not told what pressure would be expected to be an effective treatment. Furthermore, the participants were unaware as to which treatment group they were in. The prescribed IPC sessions were performed once a day over the following 7 days. Participant characteristics are provided in table 1. Urine samples were collected (midstream) 5 min before each IPC session and stored on ice after collection and subsequently stored at −80°C until analysis for markers of inflammation, oxidative stress and indirect nitric oxide/catecholamine synthesis. After completing all seven IPC treatments, the participants performed a simulated Keirin (4×30 s Wingate tests; 24 hours post) and a second and third VO2max test (48 hours, 7 days post).

Table 1

Subject characteristics


All participants visited the laboratory (~20°C; humidity 51%) on 12 separate occasions. On the first day, using a Cyclus 2 ergometer (Leipzig, Germany) and Cortex Metalyzer 3B (Biophysik, Leipzig, Germany) metabolic cart, participants performed a progressive VO2max protocol starting at 100 W with 25 W/min increments until exhaustion. A 30 s Wingate test (external drop weight equivalent to 10% body weight) performed on a Monark Ergometer (Ergomedic 894E, Vansbro, Sweden) was undertaken 24 hours later following a standardised 13 min warm-up. Participants received no verbal encouragement. Fingertip blood lactate capillary samples (LactatePro, ARKRAY, Kyoto, Japan) were collected pre, immediately and 5 min post Wingate test. Participants completed an adapted visual analogue scale (VAS) questionnaire (0–10) of fatigue22 pre, post and 30 min post. A ‘0’ represented ‘no fatigue’ while a ‘10’ represented ‘greatest fatigue possible’. While no familiarisation testing was performed before the baseline measurements, the participants were sport science students and have previously undertaken this type of testing. Nevertheless, the participants have had limited experienced with the particular testing, and we acknowledge that this is a limitation of the current study.

After baseline data collection, all participants underwent either 7 days of daily sham or IPC treatments. Wingate performance was retested 24 hours after the final IPC session during an anaerobic simulated cycling event (Keirin). The purpose of the simulated Keirin event was to understand the effect of repeated IPC on repetitive anaerobic exercise, which is common to many sports. The Keirin is a 2000 m motor-paced velodrome cycling event where competitors sprint the final 2½ laps (625 m) comprising ~30 s of anaerobic effort. A typical Keirin competition involves two morning races an hour apart with two final races 6 and 7 hours later. Using four Wingate tests, we replicated this time scale and simulated a Keirin competition. Blood lactate was collected before, immediately post and 5 min post each Wingate, while urine was collected pre, immediately post and 30 min post each Wingate test, as well as 24 hours post. Peak power (W), average power (W) and fatigue index (%) ([(peak power−finishing power)/peak power]×100) were calculated for each Wingate while participants also completed the same VAS questionnaire pre, post and 30 min post.

VO2max testing was conducted 48 hours and 7 days following the last IPC session. Each participant was asked to remain in a euhydrated state and adhere to their normal diet and activities throughout the 2 weeks of testing while also providing a training diary for the duration of the studies. Throughout the study, participants performed their normal training activities (exercising 5.1±1.7 hours/week). Participants were instructed to refrain from any form of strenuous exercise, caffeine, supplements or alcohol at least 24 hours prior to testing. For the Keirin simulation, each participant consumed water ad lib throughout the day while adhering to their normal diet.

Repeated IPC protocol

Seven days of repeated IPC was selected from previous clinically based research investigating the same principle.8 While in the supine position, a manual inflatable cuff (East Shore Medical Supply, Flushing New York, USA) was positioned on the upper leg where the adductor longus muscle attaches to the inguinal ligament. The cuff was 20 cm wide with the circumference length adjustable from 42 to 57 cm. Participants received four, 5 min episodes of IPC (220 mm Hg) or ‘sham’ treatment (20 mm Hg) interspersed with 5 min of reperfusion per episode, for each leg.5 Alternating unilateral occlusion was achieved by alternating the inflatable cuff from left to right leg (when one leg received reperfusion, the other was under ischaemia).23 Toe pulse and oxygen saturation were monitored by a handheld pulse oximeter (Vacumed, Ventura, California, USA), with pulse expected to be non-existent during ischaemia and oxygen saturation dropping below 90%.

Biochemical analysis

Urine sample preparation, analysis, volume correction, and quantification of neopterin, biopterin (oxidative stress), total neopterin (neopterin+7,8 dihydroneopterin; immune system activation) and total biopterin (biopterin+7,8 dihydrobiopterin (BH2)+tetrahydrobiopterin (BH4); nitric oxide/catecholamine synthesis) were conducted as previously described.20 21 Briefly, samples were thawed on ice and diluted 1:40 in 20 mM ammonium phosphate, pH 2.5. For total biopterin and neopterin analysis, 100 µL of diluted sample was aliquoted into a separate microcentrifuge tube. Twenty microlitres of acidic iodide (5.4% I2/10.8% KI in 1 M HCl) were added to oxidatively convert BH2 and BH4 to biopterin and 7,8-dihydroneopterin to neopterin before incubation for 15 min in the dark at room temperature. Following incubation, 10 µL of 0.6 M ascorbic acid was added to reduce the tri-iodine and 100 µL was transferred to a high-performance liquid  chromatography (HPLC) phial for strong cation exchange chromatography analysis (Luna 5 µm SCX 100 Å, 250×4.6 mm column (Phenomenex, California, USA)) on a Shimadzu LC-20A with UV detector and autosampler, RF-20A florescence detector and online degasser, with a 20 mM ammonium phosphate pH 2.5 mobile phase pumped isocratically at 1 mL/min. Biopterin and neopterin were detected at 353 nm excitation and 438 nm emission. The concentration and identity of biopterin and neopterin were compared with their corresponding standard (freshly prepared each day and spaced evenly throughout each analytical run) and quantified by peak area using the software Shimadzu Class VP.

Statistical analysis

The effects of repeated IPC on the change in aerobic and anaerobic capacity and performance, anaerobic capacity parameters during the simulated Keirin competition, and urinary analytes were tested in a linear mixed-effects model fitted with restricted maximum likelihood, conducted in the lme4 package in R V.3.1.1. Between-group and within-group changes were calculated with p values using Satterthwaite's method of denominator synthesis, conducted in the lmerTest package for R and supported with Cohen’s effect sizes (d) to evaluate the degree of change. Effect sizes were interpreted using thresholds of 0.2, 0.6, 1.2 and >2.0 for small, moderate, large and very large, respectively.24 Changes are reported in comparison with baseline for each dependent variable, with each analysed as the response variable in a separate model. All data are presented as mean±SEM, except for the anaerobic capacity and performance parameter figures where the between-participant variability has been removed according to Cousineau.25 Significance was set at p<0.05.


All 18 participants completed their respective treatment and undertook the follow-up testing; hence, all results have been analysed.

Anaerobic measures

Comparisons between pre-IPC and the best 30 s Wingate of the Keirin simulation (figure 1D–F) reveal peak power increased 11% (p<0.001, d=0.34), average power 4.3% (p=0.02, d=0.16) and fatigue index 12.1% (p=0.01, d=0.79) in the IPC group, while no changes were observed for the sham group for any of these parameters. Both groups reported a greater perceived feeling of fatigue 30 min following the first Keirin Wingate test despite no significant changes in lactate measures for either group between the pre-IPC and first Keirin Wingate test (see online supplementary table 3).

Supplementary Material

Supplementary data
Figure 1

Changes in relative VO2max (A), absolute VO2max (B), maximum aerobic power (C), peak power (D), average power (E) and fatigue index (F) for the repeated ischaemic preconditioning (IPC) and ‘sham’ groups (sham=40 min/day at 20 mm Hg, IPC=40 min/day at 220 mm Hg). Data are mean+SEM. *,#p<0.05, **,##p<0.01, ***p<0.001; *within group and #between groups.

Aerobic measures

Maximal aerobic capacity increased 9.5% 48 hours post IPC treatment with a further increase to 12.8% above baseline after an additional 7 days (p<0.01, d=0.91, moderate effect), as illustrated in figure 1A, B. The IPC treatment also showed a small (p<0.01, d=0.46) effect on increasing maximal aerobic power, which peaked 18.5% above baseline 48 hours postintervention before regressing back slightly to 16.1% 7 days later (figure 1C). No significant differences were found for maximal heart rate or respiratory exchange ratio (RER) values in either group during any of the VO2max tests (see online supplementary table 1).

Biochemical measures during IPC

Repeated IPC treatment resulted in significant changes and differences between groups in oxidative stress markers (p<0.05), with large effect sizes (d=1.91; d=1.82) for the weekly increase in biopterin and neopterin, respectively (figure 2). Both groups displayed large (d=1.91 and d=1.31) within-group reductions (47% and 36%) in total biopterin for the IPC (p<0.01) and sham (p<0.05) groups, respectively.

Figure 2

Concentration of urinary biochemical markers during 7 days of ischaemic preconditioning (IPC) for the IPC and ‘sham’ groups, including total neopterin (A), total biopterin (B), neopterin (C) and biopterin (D). *p<0.05, **p<0.01, ***p<0.001.

Keirin event simulation

Compared with the first Keirin Wingate test, the IPC group significantly increased their peak power by 8.7% in tests 3 and 4 (p<0.001, d=0.14), while there were no significant changes in the ‘sham’ group (figure 3A). Average power had a significant but trivial increase for Wingate 3 and 4 (p<0.05, d<0.10) when compared with Wingate 1 and 2 (3.2% and 3.4%, respectively) for the IPC group, but no changes were observed for the ‘sham’ group (figure 3B). While the IPC group’s fatigue index was elevated during tests 3 and 4 (p<0.01, d=0.34), only Wingate test 3 resulted in significantly greater (p<0.05, d=0.33) levels of fatigue for the ‘sham’ protocol (figure 3C).

Figure 3

Peak power (A), average power (B), fatigue index (C), total neopterin (D), total biopterin (E), neopterin (F) and biopterin (G) for the repeated ischaemic preconditioning (IPC) and ‘sham’ protocols during the simulated Keirin competition. Biochemical concentrations were compared with pre-exercise levels. Data are mean±SEM. *p<0.05, **p<0.01, ***p<0.001.

Ratings of perceived fatigue showed similar within-group trends as both groups experienced increased levels of fatigue (p<0.01) at specific time points. The ‘sham’ group had higher blood lactate concentrations prior to test 2 (p<0.05, d=0.67), while no other statistical differences were observed for either group.

Total neopterin transiently increased following Wingate 2 for the IPC group (p=0.02–0.04, d=0.39–0.42), while sham treatment resulted in sustained elevations above baseline at several time points (p<0.05, d=0.33–0.37) (figure 3D). This resulted in significant differences between groups before Wingate 3 commenced (p=0.04, d=0.35). IPC resulted in moderate total biopterin increases throughout the Keirin (p<0.05 to <0.001, d=0.66–0.77) (figure 3E), with no evident change in the ‘sham’ group (p>0.05). These changes resulted in small to moderate absolute differences between groups (p<0.05, d=0.46–0.60) throughout the simulation.

The oxidised compounds neopterin and biopterin showed markedly different responses. Neopterin significantly decreased (p<0.01 to <0.001, d=0.51–0.71) during the second half of the Keirin simulation for the IPC group compared with baseline (figure 3F), while an immediate and sustained increase was observed for the ‘sham’ group (p<0.05 to <0.001, d=0.53–0.61), which returned to baseline concentrations during Wingate 3 and 4 (figure 3F) before increasing significantly 24 hours later (p<0.01, d=1.1). Consequently, IPC resulted in significantly lower changes (p<0.05 to <0.01, d=0.54–0.66) compared with ‘sham’ throughout the Keirin simulation and 24 hours post (p<0.001, d=1.51). Biopterin significantly increased compared with baseline immediately prior to the second Wingate test (figure 3G). Concentrations remained elevated throughout with greater changes observed for the ‘sham’ group (p<0.01–0.001, d=0.73–0.81) compared with IPC (p<0.05 to p<0.001, d=0.61–0.83). This resulted in statistically greater differences between groups throughout the third Wingate (p<0.05, d=0.34–0.40) and 24 hours post (p<0.05, d=1.28).


Utilising repeated daily ischaemia and reperfusion to elicit an improvement in exercise performance has only recently been investigated8 26 without considering its use as a ‘conditioning tool’. The results suggest that seven consecutive days of IPC may provide ergogenic benefit; however, the optimal conditioning required to elicit a performance or physiological adaptation is yet to be investigated and remains a topic of interest in cardioprotection.6 The magnitude of physiological changes seen is substantially greater than reported by de Groot et al 4 and Lalonde and Curnier27 who noted that a single application of leg ischaemia improves maximal oxygen consumption by 3%, aerobic power output by 1.6% and had no change in anaerobic capacity. Our within-treatment group improvement of 12.8% in maximal oxygen consumption, 18.5% enhancement in aerobic power, and 11% and 4.3% increase in peak and average anaerobic power, respectively, looks to justify 7 days of IPC. Yet our data need to be carefully considered in relation to the recreation population recruited, their training status and the psychological factor where participants may have knowingly ‘held-back’ with the thought of four upcoming bouts. Significant elevations in peak and average power for the IPC group during the Keirin simulation, two critical determining factors in repeated bout anaerobic sport, identify the potential of repeated IPC exposure. These changes are in line with previous IPC research.28

We undertook performance testing, 24 and 48 hours post the last IPC session, as practically this fits with when athletes are likely to perform their last exercise sessions during the taper phase leading into a competition. Further study could be undertaken to ascertain if the last IPC session was conducted 72 hours prior to testing and if the repeated IPC effect would still be present.

Biochemical markers

A review of IPC literature identified a general trend towards aerobic and anaerobic performance improvement but did not elaborate on the physiological or biochemical response during each of the conditions.29 It is well-established that IPC results in cellular and genetic adaptations providing protection against hypoxic stress30 In an exercise-related context where significant elevations in oxidative stress accompany various forms of activity, the adaptation potentially developed throughout repeated IPC may have increased the cells’ ability to modulate the physiological stress response and more efficiently improve exercise capacity and performance through oxidative stress-dependent mechanisms.15 The finding that only neopterin and biopterin biomarkers were increased over the week while undertaking the IPC treatment indicates that while oxidative stress was increased, there was minimal immune system activation or nitric oxide/catecholamine synthesis. We speculate either enhanced radical scavenging, a change in the type of oxidant generated or increased oxidative stress. Considering both pterins increased and Zhou et al 31 noting oxidative stress is a necessity for IPC-induced protection, a change in the oxidant:antioxidant ratio seems like a plausible scenario. The lack of change in biomarkers of immune system activation is in further accordance with Tu et al, 15 where repetitive IPC attenuated increases in inflammatory mediators.

Repeated IPC resulted in lower levels of indirect markers of oxidative stress during the Keirin simulation. This may explain the ability to maintain a high peak power and reduce the decrement in average power over successive sprints. Increased Reactive Oxygen Species (ROS) such as those produced during high-intensity exercise32 has been shown to modulate skeletal muscle force production,33 while immune system activation is known to detrimentally affect the same mechanical properties.34 It could be postulated that the smaller perturbations in inflammation and oxidative stress experienced in the IPC group had a smaller impact on the contractile properties of the skeletal muscles, allowing for more sustainable and repeatable anaerobic performance.

Repeated IPC also resulted in a significant elevation in urinary total biopterin, suggesting increased vasodilation and sympathetic activation as previously noted.14 This may increase oxygen delivery and saturation within the exercising skeletal muscle tissue for enhancement of contractile function, which was also observed between successive bouts.35 Horiuchi et al 13 confirmed this potential theory through improved tissue oxygenation during sympathetic activation at rest and during moderate exercise following IPC. The likely increase in vasodilation and subsequent oxygen delivery in conjunction with previously reported increased blood vessel density in response to repeated IPC-initiated hypoxic conditions36 may provide an explanation for the improvement in aerobic capacity.

With the possibility that acute or singular sessions of IPC may improve physiological variables and sporting performance, coaches will likely prescribe IPC multiple times over a set period, thereby using IPC as a conditioning tool. Studies have already begun to investigate the use of IPC over extended periods from 7 days8 to 8 weeks.10 Future studies should consider investigating multiple IPC sessions per day and use of IPC over time periods longer than 7 days, with different dosages of repeated IPC. The optimal days before competition to implement repeated IPC to maximise any benefit needs to be elucidated.

Repeated IPC over seven consecutive days increased aerobic and anaerobic capacity and anaerobic exercise performance in recreational individuals. The changes were associated with modulations in specific biomarkers of oxidative stress, inflammation and nitric oxide/neurotransmitter synthesis.


  • substantially improved aerobic and anaerobic capacity

  • improvement in certain anaerobic capacity parameters over four successive anaerobic bouts

  • significant elevations in neopterin and biopterin during repeated ischaemic preconditioning application that were attenuated in conjunction with total neopterin during repeated anaerobic exercise

  • substantially elevated concentrations in total biopterin during repeated anaerobic exercise

Collectively, these findings suggest that ischaemia/reperfusion associated with alternating unilateral repeated IPC provides a physiological stimulus capable of improving both aerobic capacity and anaerobic performance while simultaneously affecting several physiological mechanisms in recreationally active participants.



  • Acknowledgements The authors would like to thank Larissa Paes for her significant contribution to the preparation of this study, Dr Geoff Shaw for his contribution towards the concept design and each of the participants for their involvement.

  • Contributors AL manuscript preparation, revision, statistics, interpretation, study design, testing.

    CP manuscript preparation, interpretation, revision, study design, testing.

    GB study design, testing, manuscript proofing.

    HF subject recruitment, testing, manuscript proofing.

    GP biochemical analysis, manuscript proofing.

    NS biochemical analysis, manuscript proofing.

    SPG study design, results interpretation, manuscript proofing.

  • Funding This research was funded by the School of Health Sciences and School of Biological Sciences, University of Canterbury, Christchurch, New Zealand.

  • Competing interests None declared.

  • Ethics approval University of Canterbury, Human Ethics Committee.

  • Provenance and peer review Not commissioned; externally peer reviewed.