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Exercise training improves function of circulating angiogenic cells in patients with chronic heart failure

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Abstract

Alterations in circulating angiogenic cells (CAC) and endothelial progenitor cells (EPC), known to contribute to endothelial repair, could explain the reversal of endothelial function in response to exercise training. Moreover, training-induced vascular remodeling might affect the acute response of EPC and CAC following a single exercise bout. We studied the impact of exercise training on CAC function and numbers of CD34+/KDR+ EPC in patients with chronic heart failure (CHF) and we assessed the effect of acute exercise on CAC and EPC in sedentary and trained patients. Twenty-one sedentary CHF patients underwent 6-month exercise training and were compared to a non-trained control group (n = 17) and 10 healthy age-matched subjects. At baseline and follow-up, flow-mediated dilation was assessed and graded exercise testing (GXT) was performed. Before and immediately after GXT, CAC migratory capacity was assessed in vitro and circulating CD34+/KDR+ EPC were quantified using flow cytometry. At baseline, CAC migration was significantly impaired in sedentary CHF patients but normalized acutely after GXT. Training corrected endothelial dysfunction, which coincided with a 77% increase in CAC migration (P = 0.0001). Moreover, the GXT-induced improvement detected at baseline was no longer observed after training. Numbers of CD34+/KDR+ EPC increased following 6-month exercise training (P = 0.021), but were not affected by GXT, either prior or post-training. In conclusion, the present findings demonstrate for the first time that exercise training in CHF reverses CAC dysfunction and increases numbers of CD34+/KDR+ EPC, which is accompanied by improvement of peripheral endothelial function. The acute exercise-induced changes in CAC function wane with exercise training, suggesting that repetitive exercise bouts progressively lead to functional endothelial repair.

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Acknowledgments

The study was supported by the University of Antwerp, the Fund for Scientific Research (FWO-Flanders) and the Institute for Science and Technology (IWT). EVC and PB are supported by FWO as PhD fellows and VC as a clinical Postdoctoral fellow. We acknowledge technical assistance from G. Frederix regarding cell culture, from J. Roeykens regarding exercise testing and from I. Goovaerts and G. Ennekens regarding endothelial function assessments.

Conflict of interest statement

The authors declare that they have no conflicts of interest.

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Authors and Affiliations

  1. Department of Cardiology, Antwerp University Hospital, Wilrijkstraat 10, 2650, Edegem, Belgium

    Emeline M. Van Craenenbroeck, Paul J. Beckers, Nadine M. Possemiers, Kurt Wuyts, Bernard P. Paelinck, Christiaan J. Vrints & Viviane M. Conraads

  2. Laboratory of Cellular and Molecular Cardiology, Antwerp University Hospital, Edegem, Belgium

    Emeline M. Van Craenenbroeck, Vicky Y. Hoymans, Christiaan J. Vrints & Viviane M. Conraads

  3. Centre for Cell Therapy and Regenerative Medicine (CCRG), Antwerp University Hospital, Edegem, Belgium

    Emeline M. Van Craenenbroeck, Vicky Y. Hoymans, Christiaan J. Vrints & Viviane M. Conraads

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  1. Emeline M. Van Craenenbroeck
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  2. Vicky Y. Hoymans
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Correspondence to Emeline M. Van Craenenbroeck.

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Van Craenenbroeck, E.M., Hoymans, V.Y., Beckers, P.J. et al. Exercise training improves function of circulating angiogenic cells in patients with chronic heart failure. Basic Res Cardiol 105, 665–676 (2010). https://doi.org/10.1007/s00395-010-0105-4

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  • DOI: https://doi.org/10.1007/s00395-010-0105-4

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