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Antioxidant status of elite athletes remains impaired 2 weeks after a simulated altitude training camp

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Abstract

Background

It has been shown that the antioxidant status was altered by the “live high-train low” (LHTL) method, however, no information is available regarding the antioxidant restoration during the recovery period.

Aim of the study

We tested the hypothesis that the antioxidant status is impaired by 18 days LHTL in elite athletes and remained altered after 14 days of recovery.

Methods

Eleven elite cross-country skiers from the French Skiing Federation were submitted to 18-day endurance training. Six (hypoxic group; HG) trained at 1,200 m and lived in hypoxia (simulated altitude of 2,500 m–3,000 m–3,500 m) and 5 (control group; CG) trained and lived at 1,200 m. Plasma levels of advanced oxidation protein products (AOPP), malondialdehydes (MDA), ferric reducing antioxidant power (FRAP), trolox equivalent antioxidant capacity (TEAC) lipid-soluble antioxidants (α-tocopherol, β-carotene and lycopene) were measured at rest, before (PRE), the first day after (POST1) and again 2 weeks (POST14) after the training. Intakes of vitamins A and E were evaluated from the dietary recording.

Results

In POST1, FRAP and TEAC decreased in both groups, however, the TEAC decrease persisted in POST14 for HG only. Lycopene and β-carotene decreased in POST1 for HG and remained lower in POST14. Finally, AOPP increased only for HG in POST1. The general decline of antioxidant status for both groups might result from insufficient intakes in vitamins A and E.

Conclusion

This is the first study to show that the antioxidant status did not return to baseline 2 weeks after 18 days of LHTL training.

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References

  1. ASFFA (2003) Compléments et suppléments pour le sportif. In: Apports nutritionnels conseillés pour la population française (3e édition). Tec et Doc (eds), Paris, pp 380–382

  2. Bailey DM, Davies B, Young IS (2001) Intermittent hypoxic training: implications for lipid peroxidation induced by acute normoxic exercise in active men. Clin Sci (Lond) 101:465–475

    Article  CAS  Google Scholar 

  3. Benzie IF, Strain JJ (1996) The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: the FRAP assay. Anal Biochem 239:70–76

    Article  CAS  Google Scholar 

  4. Brugniaux JV, Schmitt L, Robach P, Jeanvoine H, Zimmermann H, Nicolet G, Duvallet A, Fouillot JP, Richalet JP (2006) Living high-training low: tolerance and acclimatization in elite endurance athletes. Eur J Appl Physiol 96:66–77

    Article  Google Scholar 

  5. Brugniaux JV, Schmitt L, Robach P, Nicolet G, Fouillot JP, Moutereau S, Lasne F, Pialoux V, Saas P, Chorvot MC, Cornolo J, Olsen NV, Richalet JP (2006) Eighteen days of “living high, training low” stimulate erythropoiesis and enhance aerobic performance in elite middle-distance runners. J Appl Physiol 100:203–211

    Article  Google Scholar 

  6. Burke LM, Slater G, Broad EM, Haukka J, Modulon S, Hopkins WG (2003) Eating patterns and meal frequency of elite Australian athletes. Int J Sport Nutr Exerc Metab 13:521–538

    Google Scholar 

  7. Cao G, Prior RL (1998) Comparison of different analytical methods for assessing total antioxidant capacity of human serum. Clin Chem 44:1309–1315

    CAS  Google Scholar 

  8. Clarkson PM (1995) Antioxidants and physical performance. Crit Rev Food Sci Nutr 35:131–141

    Article  CAS  Google Scholar 

  9. Dragsted LO, Pedersen A, Hermetter A, Basu S, Hansen M, Haren GR, Kall M, Breinholt V, Castenmiller JJ, Stagsted J, Jakobsen J, Skibsted L, Rasmussen SE, Loft S, Sandstrom B (2004) The 6-a-day study: effects of fruit and vegetables on markers of oxidative stress and antioxidative defense in healthy nonsmokers. Am J Clin Nutr 79:1060–1072

    CAS  Google Scholar 

  10. Gore CJ, Hahn A, Rice A, Bourdon P, Lawrence S, Walsh C, Stanef T, Barnes P, Parisotto R, Martin D, Pyne D (1998) Altitude training at 2690 m does not increase total haemoglobin mass or sea level VO2max in world champion track cyclists. J Sci Med Sport 1:156–170

    Article  CAS  Google Scholar 

  11. Joanny P, Steinberg J, Robach P, Richalet JP, Gortan C, Gardette B, Jammes Y (2001) Operation Everest III (Comex’97): the effect of simulated sever hypobaric hypoxia on lipid peroxidation and antioxidant defence systems in human blood at rest and after maximal exercise. Resuscitation 49:307–314

    Article  CAS  Google Scholar 

  12. Jordan W, Cohrs S, Degner D, Meier A, Rodenbeck A, Mayer G, Pilz J, Ruther E, Kornhuber J, Bleich S (2006) Evaluation of oxidative stress measurements in obstructive sleep apnea syndrome. J Neural Transm 113:239–254

    Article  CAS  Google Scholar 

  13. Koechlin C, Couillard A, Simar D, Cristol JP, Bellet H, Hayot M, Prefaut C (2004) Does oxidative stress alter quadriceps endurance in chronic obstructive pulmonary disease? Am J Respir Crit Care Med 169:1022–1027

    Article  Google Scholar 

  14. Le Moulenc N, Deheeger M, Preziosi P, Monterio P, Valeix P, Rolland-Cachera MF, Potier de Gourcy G, Christides JP, Galan P, Hercberg S (1996) Validation du manuel photo utilisé dans l’enquête alimentaire SU.VI.MAX. Cah Nutr Diet 3:58–16

  15. Lefevre G, Beljean-Leymarie M, Beyerle F, Bonnefont-Rousselot D, Cristol JP, Therond P, Torreilles J (1998) Evaluation of lipid peroxidation by measuring thiobarbituric acid reactive substances. Ann Biol Clin (Paris) 56:305–319

    CAS  Google Scholar 

  16. Levine BD (2002) Intermittent hypoxic training: fact and fancy. High Alt Med Biol 3:177–193

    Article  Google Scholar 

  17. Levine BD, Stray-Gundersen J (1997) “Living high-training low”: effect of moderate-altitude acclimatization with low-altitude training on performance. J Appl Physiol 83:102–112

    CAS  Google Scholar 

  18. Lyan B, Zais-Braesco V, Cardinault N, Tyssandier V, Borel P, Exandre-Gouabau MC, Grolier P (2001) Simple method for clinical determination of 13 carotenoids in human plasma using an isocratic high-performance liquid chromatographic method. J Chromatogr B Biomed Sci Appl 751:297–303

    Article  CAS  Google Scholar 

  19. Moore K, Roberts LJ (1998) Measurement of lipid peroxidation. Free Radic Res 28:659–671

    Article  CAS  Google Scholar 

  20. Palazzetti S, Rousseau AS, Richard MJ, Favier A, Margaritis I (2004) Antioxidant supplementation preserves antioxidant response in physical training and low antioxidant intake. Br J Nutr 91:91–100

    Article  CAS  Google Scholar 

  21. Pialoux V, Mounier R, Brown AD, Steinback CD, Rawling JM, Poulin MJ (2009) Relationship between oxidative stress and HIF-1 alpha mRNA during sustained hypoxia in humans. Free Radic Biol Med 46:321–326

    Article  CAS  Google Scholar 

  22. Pialoux V, Mounier R, Brugniaux JV, Rock E, Mazur A, Richalet JP, Robach P, Coudert J, Fellmann N (2009) Thirteen days of “live high-train low” does not affect prooxidant/antioxidant balance in elite swimmers. Eur J Appl Physiol 106(4):517–524

    Google Scholar 

  23. Pialoux V, Mounier R, Ponsot E, Rock E, Mazur A, Dufour S, Richard R, Richalet JP, Coudert J, Fellmann N (2006) Effects of exercise and training in hypoxia on antioxidant/pro-oxidant balance. Eur J Clin Nutr 60:1345–1354

    Article  CAS  Google Scholar 

  24. Pialoux V, Mounier R, Rock E, Mazur A, Schmitt L, Richalet JP, Robach P, Brugniaux J, Coudert J, Fellmann N (2009) Effects of the ‘live high-train low’ method on prooxidant/antioxidant balance on elite athletes. Eur J Clin Nutr 63(6):756–762

    Google Scholar 

  25. Pialoux V, Mounier R, Rock E, Mazur A, Schmitt L, Richalet JP, Robach P, Coudert J, Fellmann N (2009) Effects of acute hypoxic exposure on prooxidant/antioxidant balance in elite endurance athletes. Int J Sports Med 30:87–93

    Article  CAS  Google Scholar 

  26. Robach P, Schmitt L, Brugniaux JV, Nicolet G, Duvallet A, Fouillot JP, Moutereau S, Lasne F, Pialoux V, Olsen NV, Richalet JP (2006) Living high-training low: effect on erythropoiesis and maximal aerobic performance in elite Nordic skiers. Eur J Appl Physiol 97:695–705

    Article  Google Scholar 

  27. Rousseau AS, Hininger I, Palazzetti S, Faure H, Roussel AM, Margaritis I (2004) Antioxidant vitamin status in high exposure to oxidative stress in competitive athletes. Br J Nutr 92:461–468

    Article  CAS  Google Scholar 

  28. Sen CK (2001) Antioxidants in exercise nutrition. Sports Med 31:891–908

    Article  CAS  Google Scholar 

  29. Vasankari TJ, Kujala UM, Rusko H, Sarna S, Ahotupa M (1997) The effect of endurance exercise at moderate altitude on serum lipid peroxidation and antioxidative functions in humans. Eur J Appl Physiol Occup Physiol 75:396–399

    Article  CAS  Google Scholar 

  30. Vollaard NB, Shearman JP, Cooper CE (2005) Exercise-induced oxidative stress:myths, realities and physiological relevance. Sports Med 35:1045–1062

    Article  Google Scholar 

  31. Wing SL, Askew EW, Luetkemeier MJ, Ryujin DT, Kamimori GH, Grissom CK (2003) Lack of effect of Rhodiola or oxygenated water supplementation on hypoxemia and oxidative stress. Wilderness Environ Med 14:9–16

    Google Scholar 

  32. Witko-Sarsat V, Friedlander M, Capeillere-Blandin C, Nguyen-Khoa T, Nguyen AT, Zingraff J, Jungers P, Scamps-Latscha B (1996) Advanced oxidation protein products as a novel marker of oxidative stress in uremia. Kidney Int 49:1304–1313

    Article  CAS  Google Scholar 

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Acknowledgments

The authors gratefully thank the subjects for their contribution. This study was funded by the “International Olympic Committee”, the French “Ministère des sports” and the “Direction Régionale de la Jeunesse et des Sports de la Région Auvergne”.

Conflict of interest statement

The authors declare no competing financial interests.

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Correspondence to Vincent Pialoux.

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Pialoux, V., Brugniaux, J.V., Rock, E. et al. Antioxidant status of elite athletes remains impaired 2 weeks after a simulated altitude training camp. Eur J Nutr 49, 285–292 (2010). https://doi.org/10.1007/s00394-009-0085-z

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  • DOI: https://doi.org/10.1007/s00394-009-0085-z

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